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Researchers from the UMU analyze the gene expression of porcine oviduct for its possible applications in assisted reproduction (02/11/2017)

Scientists of the Department of Cell Biology and Histology of the F. de Medicina, led by Dr. Mª José Izquierdo Rico of the research group of Dr. Manuel Avilés, and of the Department of Physiology, of F. de Veterinaria (group of the Dr. Coy), they have carried out a study on gene expression in the oviduct, a conduit that connects each ovary with the uterus, during the stern cycle of the sow for its possible applications in assisted reproduction.

The analysis, published in the journal Reproduction, Fertility and Development, has been carried out in four different phases of the sow cycle: prepubertal, preovulatory;

postovulatory and luteal, to compare the gene expression in each of them.

The results show that a total of 4929 genes are expressed, of which 86, 89 and 15 genes rise in the prepubertal, preovulatory and luteal phases with respect to the postovulatory phase.

In addition, 80, 51 and 64 fall in prepubertal, preovulatory and luteal, respectively, comparing it with the postovulatory phase.

These data were obtained by means of Microarrays, a technique that analyzes the gene expression of a tissue by hybridization.

To validate and complement the first method used, a second technique called quantitative PCR was used to analyze how a gene is expressed in real time. In a second instance, the study focused on five genes (CCK, GPX2, MUC1, PEBP4 and TAC3) for its potential involvement in reproductive processes.

Their expression was analyzed by means of two techniques: proteomics, used to detect the protein in oviductal fluid, and immunohistochemistry, to localize the expression of corresponding proteins in the oviduct.

Dr. Mª José Izquierdo Rico affirms these findings are essential to understand the mechanisms that occur in the oviduct such as fertilization or early embryonic development, being very useful in the improvement of assisted reproduction techniques.

Source: Universidad de Murcia

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